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#Question id: 16134
#SCPH01 Biochemistry
You obtain 6 BACs (of known order, as shown below) and 7 STSs (of unknown order) that derive from a region of mouse chromosome 16 whose genomic sequence has not yet been finished.
By PCR (using 20-bp primers at either end of each STS), you test each of the 6 BACs for the presence (+) or absence (-) of each of the 7 STSs. You obtain the following results:
You determine the DNA sequences of PCR products obtained at STS5 using BACs B, C, D, E, and F as PCR templates. The DNA sequences of PCR products obtained using BACs B and C are identical, but they differ by several nucleotide substitutions from the DNA sequences of PCR products obtained using BACs E and F. Oddly, PCR product obtained using BAC D appears to be a mixture of two sequences – one corresponding to the BAC B/ BAC C sequence and the other corresponding to the BAC E/ BAC F sequence. Briefly account for these findings, and explain how a single PCR assay could amplify two different sequences.
TLS Online TPP Program
#Question id: 22949
#SCPH06 I Botany
How CONSTANS gene and its encoded protein involved in translating day length into a signal that converts a vegetative shoot meristem into a floral meristem?
TLS Online TPP Program
#Question id: 10579
#SCPH06 I Botany
The following graph is growing population, which of the following represent growth pattern?
TLS Online TPP Program
#Question id: 3616
#SCPH12 I Genetics
If the parents are AO and BO genotypes for the ABO blood group, their children could include which of the following genotypes?
TLS Online TPP Program
#Question id: 266
#SCPH12 I Genetics
________ plays an important role in storing the energy needed for the unwinding of DNA during replication, recombination, repair and transcription.