TLS Online TPP Program
More Questions
TLS Online TPP Program
#Question id: 13090
#SCPH05 I Biotechnology
To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.
The distance between each tick mark placed on the line above is 100 bases in length
Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’
The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.
You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.
What is the Colony 3’s plasmid is;
TLS Online TPP Program
#Question id: 11956
#SCPH28 | Zoology
An advantage of a complete digestive system over a gastrovascular cavity is that the complete system ________.
TLS Online TPP Program
#Question id: 19213
#SCPH28 | Zoology
In Agrobacterium tumefaciens Crown gall formation is the consequence of
TLS Online TPP Program
#Question id: 925
#I Life Science/ Life Sciences Group – I-V
If an aerobic organism (e.g., the bacterium E. coli) were fed each of the following four compounds as a source of energy, the energy yield per mole from these molecules would be in the order:
TLS Online TPP Program
#Question id: 16475
#SCPH06 I Botany
Cultures that facilitate cell-to-cell interactions and signaling among cells and allow their study, are