TLS Online TPP Program

#Question id: 28023


A research problem is feasible only when:

#Research Methodology
  1. it has utility and relevance 
  2. it is researchable
  3. it is new and adds something to knowledge
  4. All of the above
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TLS Online TPP Program

#Question id: 5527

#I Life Science/ Life Sciences Group – I-V

Ectoderm cells related with anterior neural crest cells condense and form

TLS Online TPP Program

#Question id: 10281

#SCPH05 I Biotechnology

Gluconeogenesis is particularly important in plants such as the castor oil plant Ricinus communis and sunflower that store carbon in which form .

TLS Online TPP Program

#Question id: 348

#SCPH05 I Biotechnology

Sulfur is in the same column of the periodic table as oxygen, but has electronegativity similar to carbon. Compared to water molecules, molecules of H2S will ________.

TLS Online TPP Program

#Question id: 15654

#SCPH06 I Botany

You are studying a new strain of E. coli that can utilize the disaccharide melibiose very efficiently. You find that utilization depends on the enzyme melibiase, which is encoded by the gene Mel1. Mel1 is not expressed unless melibiose is present in the growth medium. Next you isolate a mutation, designated MelB–, which gives uninducible melibiase activity. Mapping experiments show that MelB– is linked to Mel1. Using an F' factor that carries the chromosomal region surrounding Mel1, you perform the following genetic tests:

                      
Describe the basic genetic properties of the MelB– mutation?

TLS Online TPP Program

#Question id: 12909

#SCPH06 I Botany

According to the diagram, arrange the steps in correct order: 


1. DNA is then digested by the Cas9 nuclease at specific points on both DNA strands. 
2. Homologous recombination to repair the break and incorporate the new DNA sequence then follows. 
3.The nuclease Cas9 is targeted to DNA by a guide RNA consisting of a specific 20 nucleotide spacer/targeting sequence (crRNA).
 4. That identifies the DNA to be modified, linked to a trans-activating (tracrRNA) sequence necessary for the recruitment and stability of Cas9 nuclease.