Assume that acid rain has lowered the pH of a particular lake to pH 5.0. What is the hydroxide ion concentration of this lake?

TLS Online TPP Program

#Question id: 19787

#Unit 13. Methods in Biology

Polymerase chain reaction is based on___

a) Thermal modulation

b) pH dependent

c) Formation of amplicons by inserting some inputs for analysing its efficiency

d) Inter-complementary primers formation

TLS Online TPP Program

#Question id: 19788

#Unit 13. Methods in Biology

Thermal modulation are used in PCR for the function of__

TLS Online TPP Program

#Question id: 19789

#Unit 13. Methods in Biology

How to determine the size of PCR product?

TLS Online TPP Program

#Question id: 19790

#Unit 13. Methods in Biology

What will be the major difference between the PCR and replication is?

TLS Online TPP Program

#Question id: 19791

#Unit 13. Methods in Biology

Which of the following statements about polymerase chain reaction is correct?

a) PCR denaturation temperature calculated by using Y = X (1+E)n

b) Size of the primer negatively affects the efficiency of PCR

c) Standard PCR doesn’t efficiently amplify sequence much longer than about 5kb.

d) Primer annealing of PCR at 550C for 1.5 min.

e) Time taken for each cycle of PCR considerably longer than 3 min.

TLS Online TPP Program

#Question id: 19840

#Unit 13. Methods in Biology

Match the following source of thermostable DNA polymerase with proofreading (3’−5’ exonuclease) activity;

DNA Polymerase	Sources
A- Tma	i) Pyrococcus furiosus
B- Deep VentTM	ii) Pyrococcus woesi
C- Tli	iii)  Thermotoga maritima
D- Pfu	iv) Pyrococcus sp.
E- Pwo	v) Thermococcus litoralis