Soon after the coelom begins to form, a researcher injects a dye into the coelom of a deuterostome embryo. Initially, the dye should be able to flow directly into the

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#Question id: 19791

#Unit 13. Methods in Biology

Which of the following statements about polymerase chain reaction is correct?

a) PCR denaturation temperature calculated by using Y = X (1+E)n

b) Size of the primer negatively affects the efficiency of PCR

c) Standard PCR doesn’t efficiently amplify sequence much longer than about 5kb.

d) Primer annealing of PCR at 550C for 1.5 min.

e) Time taken for each cycle of PCR considerably longer than 3 min.

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#Question id: 19840

#Unit 13. Methods in Biology

Match the following source of thermostable DNA polymerase with proofreading (3’−5’ exonuclease) activity;

DNA Polymerase	Sources
A- Tma	i) Pyrococcus furiosus
B- Deep Vent™	ii) Pyrococcus woesi
C- Tli	iii) Thermotoga maritima
D- Pfu	iv) Pyrococcus sp.
E- Pwo	v) Thermococcus litoralis

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#Question id: 19841

#Unit 13. Methods in Biology

Which of the following is true regarding Reverse transcriptase ?

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#Question id: 19842

#Unit 13. Methods in Biology

Primer-Dimer leads to the formation of ___

a) Inter-complemetary primers

b) Hybrid 

c) Amplicons

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#Question id: 19843

#Unit 13. Methods in Biology

Real time PCR analyzed various steps such as__

a) Denaturation

b) Annealing

c) Phosphorylation

d) Amplification

e) Methylation

f) Extension

Which of the following steps are correctly analyzed by real time PCR?

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#Question id: 19844

#Unit 13. Methods in Biology

Match the following column, which correctly represent the qPCR done by three methods and measure such as;

COLUMN I	COLUMN II
A) SYBR green	i) Annealing
B) Taqman Assay	ii) Denaturation
C) Molecular beacon	iii) pH
	iv) Amplification
	v) Extension