What will be the major difference between the PCR and replication is?

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#Question id: 19883

#Unit 13. Methods in Biology

Which of the following statements are true regarding rDNA technology?

a) rDNA technology is used to obtain large number of copies of specific DNA fragments

b) rDNA technology is used to obtain large quantities of the protein produced by the concerned gene

c) rDNA technology is used to integrate gene of interest into chromosomes where it express itself

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#Question id: 19884

#Unit 13. Methods in Biology

The first successful transformation of rDNA molecules into a bacterium was carried out by

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#Question id: 19885

#Unit 13. Methods in Biology

The plasmid used by Cohen and Boyer fr their transformation experiment was

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#Question id: 19886

#Unit 13. Methods in Biology

Gene cloning refers to the

I) Production of large number of copies of the gene being cloned

II) Production of asexual progeny from a single individual or a cell 

III) Mechanism of intake of DNA fragments from the surrounding medium by a cell

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#Question id: 19887

#Unit 13. Methods in Biology

Paul Berg’s gene splicing experiment created the first rDNA molecules which was a

TLS Online TPP Program

#Question id: 19888

#Unit 13. Methods in Biology

There are some typical enzyme used in the manipulation of nucleic acid;

ENZYMES	USE IN NUCLEIC ACID MANIPULATION
A) klenow	i) DNA-dependent RNA polymerase
B) T4 DNA polymerase	ii) Single-strand specific nuclease
C) Taq DNA polymerase	iii) DNA pol I lacks 5’→ 3’ exonuclease activity
D) T7 RNA polymerase	iv) Thermostable DNA polymerase used in PCR
E) S1 nuclease	v) Lack  5’→ 3’ exonuclease activity