TLS Online TPP Program

#Question id: 3854


In prokaryotes, the presence of a protein called rho will:

#Unit 3. Fundamental Processes
  1. cause RNA to continue production

  2. cause the termination of the process

  3. will do nothing

  4. none of these

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TLS Online TPP Program

#Question id: 19791

#Unit 13. Methods in Biology

Which of the following statements about polymerase chain reaction is correct?
a) PCR denaturation temperature calculated by using Y = X (1+E)n
b) Size of the primer negatively affects the efficiency of PCR
c) Standard PCR doesn’t efficiently amplify sequence much longer than about 5kb.
d) Primer annealing of PCR at 550C for 1.5 min.
e) Time taken for each cycle of PCR considerably longer than 3 min.

TLS Online TPP Program

#Question id: 19840

#Unit 13. Methods in Biology

Match the following source of thermostable DNA polymerase with proofreading (3’−5’ exonuclease) activity;

                 DNA Polymerase

                        Sources

 

 A- Tma

 

i) Pyrococcus furiosus

 

 B- Deep VentTM

 

ii) Pyrococcus woesi

 

 C- Tli

 

iii)  Thermotoga maritima

 

 D- Pfu

 

iv) Pyrococcus sp.

 

 E- Pwo

 

v) Thermococcus litoralis

TLS Online TPP Program

#Question id: 19841

#Unit 13. Methods in Biology

Which of the following is true regarding Reverse transcriptase ?

TLS Online TPP Program

#Question id: 19842

#Unit 13. Methods in Biology

Primer-Dimer leads to the formation of ___
a) Inter-complemetary primers
b) Hybrid 
c) Amplicons

TLS Online TPP Program

#Question id: 19843

#Unit 13. Methods in Biology

Real time PCR analyzed various steps such as__
a) Denaturation
b) Annealing
c) Phosphorylation
d) Amplification
e) Methylation
f) Extension
Which of the following steps are correctly analyzed by real time PCR?

TLS Online TPP Program

#Question id: 19844

#Unit 13. Methods in Biology

Match the following column, which correctly represent the qPCR done by three methods and measure such as;

                  COLUMN I

                    COLUMN II

A) SYBR green

i) Annealing

B) Taqman Assay

ii) Denaturation

C) Molecular beacon

iii) pH

 

iv) Amplification

 

v) Extension