TLS Online TPP Program

#Question id: 12721


There are the five groups of late embryogenesis abundant (LEA) proteins found in plants

Match the following groups of LEA protein with its functional information;

 Group (family name)

                           Functional information

 

I) Group 1

   (D-19 family)

 

 

a) It is an abundant protein in cotton embryos, Each putative dimer of this group may bind as many as ten inorganic phosphates and their counterions

 

 

II) Group 2

    (D-11 family)

 

b) Possibly functions in ion sequestration to protect cytosolic metabolism When LE25 is expressed in yeast, it confers salt and freezing tolerance

 

 

III) Group 3

     (D-7 family)

 

c) In tomato, a gene encoding a similar protein is expressed in response to nematode feeding

 

 

IV) Group 4

    (D-95 family)

 

d) Contains more water of hydration than typical globular proteins Overexpression confers water deficit tolerance on yeast cells

 

 

V) Group 5

    (D-113 family)

 

e) Often localized to the cytoplasm or nucleus More acidic members of the family are associated with the plasma membrane May act to stabilize macromolecules at low water potential

 

#Unit 6. System Physiology – Plant
  1. I-d,  II-e,  III-a,  IV-c,  V-b
  2. I-d,  II-e,  III-a,  IV-b,  V-c
  3. I-e,  II-d,  III-a,  IV-b,  V-c
  4. I-c,  II-a,  III-e,  IV-b,  V-d
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TLS Online TPP Program

#Question id: 19790

#Unit 13. Methods in Biology

What will be the major difference between the PCR and replication is?

TLS Online TPP Program

#Question id: 19791

#Unit 13. Methods in Biology

Which of the following statements about polymerase chain reaction is correct?
a) PCR denaturation temperature calculated by using Y = X (1+E)n
b) Size of the primer negatively affects the efficiency of PCR
c) Standard PCR doesn’t efficiently amplify sequence much longer than about 5kb.
d) Primer annealing of PCR at 550C for 1.5 min.
e) Time taken for each cycle of PCR considerably longer than 3 min.

TLS Online TPP Program

#Question id: 19840

#Unit 13. Methods in Biology

Match the following source of thermostable DNA polymerase with proofreading (3’−5’ exonuclease) activity;

                 DNA Polymerase

                        Sources

 

 A- Tma

 

i) Pyrococcus furiosus

 

 B- Deep VentTM

 

ii) Pyrococcus woesi

 

 C- Tli

 

iii)  Thermotoga maritima

 

 D- Pfu

 

iv) Pyrococcus sp.

 

 E- Pwo

 

v) Thermococcus litoralis

TLS Online TPP Program

#Question id: 19841

#Unit 13. Methods in Biology

Which of the following is true regarding Reverse transcriptase ?

TLS Online TPP Program

#Question id: 19842

#Unit 13. Methods in Biology

Primer-Dimer leads to the formation of ___
a) Inter-complemetary primers
b) Hybrid 
c) Amplicons

TLS Online TPP Program

#Question id: 19843

#Unit 13. Methods in Biology

Real time PCR analyzed various steps such as__
a) Denaturation
b) Annealing
c) Phosphorylation
d) Amplification
e) Methylation
f) Extension
Which of the following steps are correctly analyzed by real time PCR?