Heat-loss mechanism:

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#Question id: 19840

#Unit 13. Methods in Biology

Match the following source of thermostable DNA polymerase with proofreading (3’−5’ exonuclease) activity;

DNA Polymerase	Sources
A- Tma	i) Pyrococcus furiosus
B- Deep VentTM	ii) Pyrococcus woesi
C- Tli	iii)  Thermotoga maritima
D- Pfu	iv) Pyrococcus sp.
E- Pwo	v) Thermococcus litoralis

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#Question id: 19841

#Unit 13. Methods in Biology

Which of the following is true regarding Reverse transcriptase ?

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#Question id: 19842

#Unit 13. Methods in Biology

Primer-Dimer leads to the formation of ___

a) Inter-complemetary primers

b) Hybrid 

c) Amplicons

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#Question id: 19843

#Unit 13. Methods in Biology

Real time PCR analyzed various steps such as__

a) Denaturation

b) Annealing

c) Phosphorylation

d) Amplification

e) Methylation

f) Extension

Which of the following steps are correctly analyzed by real time PCR?

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#Question id: 19844

#Unit 13. Methods in Biology

Match the following column, which correctly represent the qPCR done by three methods and measure such as;

COLUMN I	COLUMN II
A) SYBR green	i) Annealing
B) Taqman Assay	ii) Denaturation
C) Molecular beacon	iii) pH
	iv) Amplification
	v) Extension

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#Question id: 19845

#Unit 13. Methods in Biology

SYBR Green qPCR method is based on____I_______ with the _____II______ relationship.