In mammals, the bipotential gonad appears to be moved into the male pathway (testis development) by the Sry gene (on the Y chromosome), which triggers the activity of Sox9.

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#Unit 13. Methods in Biology

The more metabolically active cells that are present in the culture, the more formazan will be generated, and so this assay provides a readout of the number of live cells as a function of time

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#Unit 13. Methods in Biology

 The MTT assay can measure cell proliferation or cell death.

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#Unit 13. Methods in Biology

bromodeoxyuridine (BrdU) is rapidly phosphorylated and incorporated in its place into newly synthesized DNA hence BrdU is used to detect replicating cells

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#Unit 13. Methods in Biology

BrdU can CAUSE light induced cell death. 

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#Unit 13. Methods in Biology

If cells that have incorporated a high level of BrdU are exposed to light, they will photolyse; this has been used to selectively kill newly dividing cells.

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#Unit 13. Methods in Biology

Propidium iodide is a fluorescent dye with a flat, planar structure that slides between the rungs of (or intercalates into) the DNA ladder in a quantitative manner frequently used in FACS.