TLS Online TPP Program

#Id: 10192


cZ is thought to be less active and relatively more stable than tZ and iP because of its low affinity for CKX. cZ and its conjugates are more abundant than tZ‐ and iP‐type CKs in Z. mays, O. sativa, chickpea (Cicer arietinum) and a moss (Physcomitrella patens)

#Unit 6. System Physiology – Plant #Cytokinins #Part B Pointers
More Pointers
TLS Online TPP Program

#Id: 5065

#Unit 3. Fundamental Processes

SeqA mutants reduce the delay in remethylation at both oriC and dnaA. As a result, they initiate DNA replication too soon, thereby accumulating an excessive number of origins.

TLS Online TPP Program

#Id: 5066

#Unit 3. Fundamental Processes

SeqA is part of a negative regulatory circuit that prevents origins from being remethylated.

TLS Online TPP Program

#Id: 5067

#Unit 3. Fundamental Processes

Mutations in dnaA render replication asynchronous, which suggests that DnaA could be the “titrator” or “clock” that measures the number of origins relative to cell mass.

TLS Online TPP Program

#Id: 5068

#Unit 3. Fundamental Processes

dat locus is regulatory
In particular, a locus called dat has a large concentration of DnaA-binding sites. It binds about 80 more DnaA molecules than the origin.
Deletion of dat causes initiation to occur more frequently. This significantly increases the amount of DnaA available to the origin.

TLS Online TPP Program

#Id: 5069

#Unit 3. Fundamental Processes

DnaA promotes replication
SeqA delays new round of replication by inhibiting dam methylase binding
Dam methylase provide substrate for DnaA

TLS Online TPP Program

#Id: 5070

#Unit 3. Fundamental Processes

DnaA mutants make replication asynchronous
SeqA mutant cells don’t delay rereplication for 13 minutes
Dam methylase mutants stops rereplication
SeqA-/dam+ accumulation of methylated products
SeqA+/dam- accumulation of hemimethylated products
SeqA-/dam- accumulation of hemimethylated products