P-450 enzymes, modify benzo(a)pyrene in a series of reactions, producing 7,8-diol-9,10-epoxide, a highly potent mutagenic species

TLS Online TPP Program

#Id: 690

#Unit 3. Fundamental Processes

t-loop is formed by the 3’-ssDNA end of the telomere invading the dsDNA region of the telomere

TLS Online TPP Program

#Id: 691

#Unit 3. Fundamental Processes

TRF2 is capable of directing t-loop formation with purified telomere DNA.

TLS Online TPP Program

#Id: 692

#Unit 3. Fundamental Processes

Mutants that used to analyse Replication Machinery are usually Conditional Lethals

TLS Online TPP Program

#Id: 693

#Unit 3. Fundamental Processes

Permissive conditions are those whose in which mutants performs replication

TLS Online TPP Program

#Id: 694

#Unit 3. Fundamental Processes

Quick-stop mutants are defective in the components of the replication apparatus and cease replication immediately upon a temperature increase.

TLS Online TPP Program

#Id: 695

#Unit 3. Fundamental Processes

1. DNA polymerase I is a single polypeptide of 103 kD. 

2. The chain can be cleaved into two parts by proteolytic treatment. The larger cleavage product (68 kD) is called the Klenow fragment. It is used in synthetic reactions in vitro. 

3. It contains the polymerase and the proofreading 3’–5’ exonuclease activities.