SOC1 and AP1 triggers LFY gene expression

TLS Online TPP Program

#Id: 8546

#Unit 1. Molecules and their Interaction Relevant to Biology

The extent of irreversible inhibition of the enzyme is dependent upon the reaction rate constant (and hence time) for covalent bond formation and upon the amount of inhibitor present.

TLS Online TPP Program

#Id: 8547

#Unit 1. Molecules and their Interaction Relevant to Biology

The irreversible inhibitors cannot be removed by simple physical techniques such as dialysis, is to reduce the amount of enzyme available for reaction.

TLS Online TPP Program

#Id: 8548

#Unit 1. Molecules and their Interaction Relevant to Biology

TLS Online TPP Program

#Id: 8549

#Unit 1. Molecules and their Interaction Relevant to Biology

The irreversible inhibition involves reactions with a functional group, such as hydroxyl or sulphydryl, or with a metal atom in the active site or a distinct allosteric site.

TLS Online TPP Program

#Id: 8550

#Unit 1. Molecules and their Interaction Relevant to Biology

diisopropylphosphofluoridate, reacts with a serine OH group in the active site of esterases such as acetylcholinesterase/chymotrypsin, whilst the organomercury compound p-hydroxymercuribenzoate reacts with a cysteine group, in both cases resulting in covalent bond formation and enzyme inhibition.

TLS Online TPP Program

#Id: 8551

#Unit 1. Molecules and their Interaction Relevant to Biology

di-isopropylphosphofluoridate (DFP), phenylmethyl sulphonylfluoride

(PMSF) and tosylphenylalanyl-chloromethylketone (TPCK) (all serine protease inhibitors); 

iodoacetate and cystatin (thiol protease inhibitors); 

pepstatin (aspartic protease inhibitor); 

EDTA and 1,10-phenanthroline (metalloprotease inhibitors); and amastatin and bestatin (exopeptidase inhibitors).