Nurturing Life Sciences
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#Unit 13. Methods in Biology
Tritiated (3H) Thymidine uptake used to assess Cell Division based on radioactivity measurement by scintillation counting.
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Tetrazolium compound MTT (3-(4,5-Dimethylthiazol- 2-yl)-2,5-diphenyltetrazolium bromide MTT Assay is calorimetric assay to detect cell division
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The more metabolically active cells that are present in the culture, the more formazan will be generated, and so this assay provides a readout of the number of live cells as a function of time
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The MTT assay can measure cell proliferation or cell death.