TLS Online TPP Program

#Id: 774

#Unit 3. Fundamental Processes

RNA polymerase starts most transcripts with an A (75%) or G

TLS Online TPP Program

#Id: 775

#Unit 3. Fundamental Processes

The sigma cycle. RNA polymerase binds to the promoter causing local melting of the DNA. As the polymerase moves elongating the RNA, the s-factor dissociates and joins with a new core polymerase to initiate another RNA chain.

TLS Online TPP Program

#Id: 776

#Unit 3. Fundamental Processes

A rifampicin-sensitive bacterium with the b-subunit from a rifampicin-resistant bacterium, the resulting polymerase was antibiotic-resistant.

TLS Online TPP Program

#Id: 777

#Unit 3. Fundamental Processes

FRET allows the detection of position of sigma relative to a site on the DNA to be measured with using separation techniques that might displace sigma from the core enzyme

 

TLS Online TPP Program

#Id: 778

#Unit 3. Fundamental Processes

Termination requires that all hydrogen bonds holding the RNA–DNA hybrid together must be broken, after which the DNA duplex reforms.

TLS Online TPP Program

#Id: 779

#Unit 3. Fundamental Processes

Transcription termination sites are often best characterized in vitro.