TLS Online TPP Program

#Id: 2853

#Unit 13. Methods in Biology

Molecular (sub-)structures responsible for interaction with electromagnetic radiation are called chromophores.

TLS Online TPP Program

#Id: 2854

#Unit 13. Methods in Biology

In proteins, there are three types of chromophores relevant for UV/Vis spectroscopy:

peptide bonds (amide bond);

certain amino acid side chains (mainly tryptophan and tyrosine); and

certain prosthetic groups and coenzymes (e.g. porphyrine groups such as in haem).

TLS Online TPP Program

#Id: 2855

#Unit 13. Methods in Biology

The presence of several conjugated double bonds in organic molecules possess  p ®p* transitions in the UV/Vis range of the electromagnetic spectrum. Such molecules are very useful tools in colorimetric applications.

TLS Online TPP Program

#Id: 2856

#Unit 13. Methods in Biology

Common colorimetric and UV absorption assays

TLS Online TPP Program

#Id: 2857

#Unit 13. Methods in Biology

Concentration of protein can be analyzed by to method constructive and destructive method .

TLS Online TPP Program

#Id: 2858

#Unit 13. Methods in Biology

Destructive method uses Folin reagent, Biuret, BCA, Coomassie Brilliant Blue and give absorbance at 660, 540, 562, 595 nm of wavelength respectively.