X-Linked Dominant Trait

a. Both males and females are usually affected; often, more females than males are affected.

b. Does not skip generations. Affected sons must have an affected mother; affected daughters must have either an affected mother or an affected father.

c. Affected fathers pass the trait to all their daughters.

d. Affected mothers (if heterozygous) pass the trait to half of their sons and half of their daughters.

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#Id: 4664

#Unit 13. Methods in Biology

BrdU is incorporated into the newly synthesized DNA, and is then detected with fluorescently labeled anti-BrdU antibodies

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#Unit 13. Methods in Biology

Caspases western blotting assay is also used to detect apoptosis

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#Unit 13. Methods in Biology

The total concentration of antibody in the equilibrium dialysis chamber is known, the equilibrium equation can be rewritten as:

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#Unit 13. Methods in Biology

Scatchard plots are based on repeated equilibrium dialyses with a constant concentration of antibody and varying concentration of ligand. 

If all antibodies have the same affinity, then a Scatchard plot yields a straight line with a slope of Ka. The x intercept is n, the valency of the antibody, which is 2 for IgG and other divalent Igs. For IgM, which is pentameric, n  10, and for dimeric IgA, n  4. In this graph, antibody #1 has a higher affinity than antibody #2. .  

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#Unit 13. Methods in Biology

If the antibody preparation is polyclonal and has a range of affinities, Scatchard plot yields a curved line whose slope is constantly changing. The average affinity constant K0 can be calculated by determining the value of Ka when half of the binding sites are occupied (i.e., when r  1 in this example). In this graph, antiserum #3 has a higher affinity (K0  2.4  108) than antiserum #4 (K0  1.25  108)

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#Unit 13. Methods in Biology

The bacterium Streptococcus pyogenes, for example, expresses cell-wall proteins called M antigens. Antibodies produced to streptococcal M antigens have been shown to cross-react with several myocardial and skeletal muscle proteins and have been implicated in heart and kidney damage following streptococcal infections.