Deletion plays a key role in gene trafficking.which is the movement of genes to new sites in the genome.

TLS Online TPP Program

#Id: 750

#Unit 3. Fundamental Processes

rNTPs are present at approximately 10-fold higher concentration in the cell, they are incorporated at a rate that is more than 1000-fold lower than dNTPs.

TLS Online TPP Program

#Id: 751

#Unit 3. Fundamental Processes

The palm domain is composed of a b sheet and contains the primary elements of the catalytic site.

TLS Online TPP Program

#Id: 752

#Unit 3. Fundamental Processes

DNA polymerase binds two divalent metal ions (typically Mg2+ orZn2+) that alter the chemical environment around the correctly base-paired dNTP and the 3’-OH of the primer

TLS Online TPP Program

#Id: 753

#Unit 3. Fundamental Processes

One metal ion generate a 3’O- that is primed for the nucleophilic attack of the a-phosphate of the incoming dNTP.

TLS Online TPP Program

#Id: 754

#Unit 3. Fundamental Processes

The second metal ion coordinates the negative charges of the b-phosphate and g-phosphate of the dNTP and stabilizes the pyrophosphate produced by joining the primer and the incoming nucleotide

TLS Online TPP Program

#Id: 755

#Unit 3. Fundamental Processes

DNA polymerase “grips” the template and the incoming nucleotide when a correct base pair is made via Arg, Lys and Tyr residue in its alpha helix