There are three type of Splicing Machinery

 

TLS Online TPP Program

#Id: 784

#Unit 3. Fundamental Processes

Misalignment of RNA combined with the unusually weak RNA–DNA hybrid formed from the rU-dA RNA–DNA base pairs resulting from the stretch of U residues, destabilize the elongation complex.

TLS Online TPP Program

#Id: 785

#Unit 3. Fundamental Processes

Termination efficiency can vary from 2% to 90%, Point mutations reduce termination efficiency usually occur within the stem region of the hairpin, replacing GC base pairs with weaker AT base pairs

 

TLS Online TPP Program

#Id: 786

#Unit 3. Fundamental Processes

Readthrough transcripts refer to the fraction of transcripts that are not stopped by the terminator. 

TLS Online TPP Program

#Id: 787

#Unit 3. Fundamental Processes

Termination is prevented by specific ancillary factors that interact with RNA and/or RNA polymerase, a situation referred to as antitermination. 

TLS Online TPP Program

#Id: 788

#Unit 3. Fundamental Processes

Transcription initiation and termination, both require breaking of hydrogen bonds.

TLS Online TPP Program

#Id: 789

#Unit 3. Fundamental Processes

Rho factor is an essential protein in E. coli that causes transcription termination, accounts for almost half of E. coli terminators.