Sigma Region 3/4 linker mimics RNA

TLS Online TPP Program

#Id: 2793

#Unit 13. Methods in Biology

The length of column containing one theoretical plate is referred to as the plate height, H, which has units of length normally in micrometers.

TLS Online TPP Program

#Id: 2794

#Unit 13. Methods in Biology

Plate height is simply related to the width of the analyte peak, expressed in terms of its standard deviation s , and the distance it travelled within the column, x. Specifically

TLS Online TPP Program

#Id: 2795

#Unit 13. Methods in Biology

The smaller the value of H and the greater the value of N, the more efficient is the column in separating a mixture of analytes.

TLS Online TPP Program

#Id: 2796

#Unit 13. Methods in Biology

The number of theoretical plates in the whole column of length L is equal to L divided by the plate height:

TLS Online TPP Program

#Id: 2797

#Unit 13. Methods in Biology

Relationship between the number of theoretical plates (N) and the shape of the analyte peak.

TLS Online TPP Program

#Id: 2798

#Unit 13. Methods in Biology

The precise relationship between the three factors and plate height is expressed by the van Deemter equation which is

where ux is the flow rate of the eluent and

A, B and C are constants for a particular column and stationary phase relating to multiple paths, longitudinal diffusion and equilibration time respectively