Agarose gels are formed  by suspending dry agarose in aqueous buffer, then boiling the mixture until a clear solution forms. 

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#Id: 4769

#Unit 5. Developmental Biology

Specification of the MS cell begins with maternal SKN-1 activating the genes encoding GATA transcription factors MED-1 and MED-2

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#Id: 4770

#Unit 5. Developmental Biology

The POP-1 encodes the TCF protein that binds β-catenin & blocks the pathway to the E fate by inhibiting endodermal transcription factors (END-1) in the prospective MS cell

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#Id: 4771

#Unit 5. Developmental Biology

POP-1 activates the TBX-35 gene.

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#Id: 4772

#Unit 5. Developmental Biology

The TBX-35 transcription factor activates mesodermal genes in the MS cell, including pha-4 in the pharynx and hlh-1 in muscles.

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#Id: 4773

#Unit 5. Developmental Biology

TBX-35 inhibits pal-1 gene expression, preventing the MS cell from acquiring the C-blastomere fates.

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#Id: 4774

#Unit 5. Developmental Biology

At the 4-cell stage, the EMS cell requires a signal from its neighbor (and sister cell), the P2 blastomere.