Photopolymerization is an alternative method that can be used to polymerize acrylamide gels. Ammonium persulfate and TEMED are replaced by riboflavin and when the gel is poured it is placed in front of a bright light for 2–3 h.

TLS Online TPP Program

#Id: 7429

#Unit 5. Developmental Biology

High PSF to food ratio is upregulate the translation of the PKA catalytic subunit PKA-C.

TLS Online TPP Program

#Id: 7430

#Unit 5. Developmental Biology

A second glycoprotein secreted during starvation is CMF (conditioned medium factor)

TLS Online TPP Program

#Id: 7431

#Unit 5. Developmental Biology

CMF stimulates gene expression in parallel with PSF, and both signals potentiate cAMP signalling by inducing genes involved in cAMP synthesis and detection

TLS Online TPP Program

#Id: 7432

#Unit 5. Developmental Biology

Newly activated genes are:

Adenylate cyclase A (ACA)

PKA

cAMP receptors (cARs)

Extracellular cAMP phosphodiesterase PdsA

An intracellular cAMP phosphodiesterase RegA

TLS Online TPP Program

#Id: 7433

#Unit 5. Developmental Biology

Once all proteins are functioning, a few starving cells start to emit cAMP pulses.

TLS Online TPP Program

#Id: 7434

#Unit 5. Developmental Biology

Chemotaxis of Dictyostelium myxamoebae is a result of spiral waves of cAMP.