TLS Online TPP Program

#Id: 432

#Unit 2. Cellular Organization

Core DNA invariant length of 146 bp contains core and linker histones so Mnase cuts after H1 binding site. 

TLS Online TPP Program

#Id: 433

#Unit 2. Cellular Organization

The nucleosome is a disc with DNA organized into 12/3 turns around the surface.

TLS Online TPP Program

#Id: 434

#Unit 2. Cellular Organization

The nucleosome is a disc of 11 nm diameter and 6 nm height. 2πrh is surface are and πr2h is volume of nucleosome

 

TLS Online TPP Program

#Id: 435

#Unit 2. Cellular Organization

The nucleosome consists of approximately equal masses of DNA and histones (including H1). The predicted mass of the nucleosome is 262 kD.

TLS Online TPP Program

#Id: 436

#Unit 2. Cellular Organization

The four core histones are present in equal amounts in the cell. H1 is half as abundant as the other histones, which is consistent with the finding that only one molecule of H1 can associate with a nucleosome.

TLS Online TPP Program

#Id: 437

#Unit 2. Cellular Organization

One turn around the nucleosome takes 80 bp of DNA