Gradient Gels electrophoresis are performed using gradient of  acrylamide Concentration that varies uniformly from, typically, 5% at the top of the gel to 25% acrylamide at the bottom of the gel.

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#Id: 4834

#Unit 13. Methods in Biology

A type I error is often called a false positive (an event that shows that a given condition is present when it is absent).

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#Unit 13. Methods in Biology

Example of type I error - In words of community tales, a person may see the bear when there is none (raising a false alarm) where the null hypothesis (H₀) contains the statement: “There is no bear”.

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#Unit 13. Methods in Biology

The type I error significance level or rate level is the probability of refusing the null hypothesis given that it is true.

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#Id: 4838

#Unit 13. Methods in Biology

It is represented by Greek letter α (alpha) and is also known as alpha level.

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#Unit 13. Methods in Biology

Usually, the significance level or the probability of type i error is set to 0.05(5%), assuming that it is satisfactory to have a 5% probability of inaccurately rejecting the null hypothesis.

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#Id: 4840

#Unit 13. Methods in Biology

A type II error appears when the null hypothesis is false but mistakenly fails to be refused.