IEF is a highly sensitive analytical technique and is particularly useful for studying  microheterogeneity in a protein. For example, a protein may show a single band on an SDS gel but may show three bands on an IEF gel. This may occur, for example, when a protein exists in mono-, di- and tri-phosphorylated forms. 

TLS Online TPP Program

#Id: 10288

#Unit 4. Cell Communication and Cell Signaling

TLS Online TPP Program

#Id: 10289

#Unit 4. Cell Communication and Cell Signaling

T-cell lineage

TLS Online TPP Program

#Id: 10290

#Unit 4. Cell Communication and Cell Signaling

T-Cell Development

1.Commitment

2.antigen receptor gene rearrangements

3.The selection and expansion of cells (β-selection

TLS Online TPP Program

#Id: 10291

#Unit 4. Cell Communication and Cell Signaling

The second phase of T-cell development

1.Positive selection: Selection for those cells whose T-cell receptors engage self-MHC.

2.Negative selection: Selection against those cells whose T-cell receptors bind too strongly to self-peptide/MHC combinations.

3.Lineage commitment: Commitment of thymocytes to effector cell lineages, including CD4 helper or CD8 cytotoxic populations

TLS Online TPP Program

#Id: 10292

#Unit 4. Cell Communication and Cell Signaling

TLS Online TPP Program

#Id: 10293

#Unit 4. Cell Communication and Cell Signaling