Pulsed-Field Gel Electrophoresis involves electrophoresis in agarose, where two electric fi elds are applied alternately at different angles for defined time periods (e.g. 60 s)

TLS Online TPP Program

#Id: 8589

#Unit 2. Cellular Organization

Lamins are reversibly disassembled by phosphorylation during mitosis

TLS Online TPP Program

#Id: 8590

#Unit 2. Cellular Organization

Mitotic CDKs drive phosphorylation of lamins A, B, and C results in disassembly of the intermediate filament lattice into lamin dimers.

TLS Online TPP Program

#Id: 8591

#Unit 2. Cellular Organization

Due to C-terminal prenylation of lamin B, 

lamin B dimers remain associated with the nuclear

membrane

TLS Online TPP Program

#Id: 8592

#Unit 2. Cellular Organization

During telophase, de-phosphorylation by specific phosphatases promotes lamin reassembly, which is critical to re-formation of a nuclear envelope around the daughter chromosomes.

TLS Online TPP Program

#Id: 8593

#Unit 2. Cellular Organization

Laminopathies 

Mutation in lamin A

TLS Online TPP Program

#Id: 8594

#Unit 2. Cellular Organization

Emery-Dreifuss muscular dystrophy (EDMD)

mutations in emerin, a nesprin and a SUN protein