The ‘Wallace rule’ (Lay Thein & Wallace 1986) is used to determine the appropriate stringency wash temperature: 

Tm = 4 × (number of GC base pairs) + 2 × (number of AT base pairs

TLS Online TPP Program

#Id: 9477

#Unit 4. Cell Communication and Cell Signaling

All SOCS proteins contains:

SH2 domain

SOCS box

TLS Online TPP Program

#Id: 9478

#Unit 4. Cell Communication and Cell Signaling

SOCS box, 

Recruits E3 ubiquitin ligases that polyubiquitinylate the receptor and the JAK kinase & marks the proteins for destruction by a proteasome

TLS Online TPP Program

#Id: 9479

#Unit 4. Cell Communication and Cell Signaling

SOCS-1, binds to the critical phosphotyrosine in the activation loop of an activated JAK2 kinase, inhibiting its catalytic activity

TLS Online TPP Program

#Id: 9480

#Unit 4. Cell Communication and Cell Signaling

TLS Online TPP Program

#Id: 9481

#Unit 4. Cell Communication and Cell Signaling

TLS Online TPP Program

#Id: 9604

#Unit 4. Cell Communication and Cell Signaling

NGF, PDGF, FGF, EGF, insulin are RTK ligands, which trigger proliferation of cells.