Probe length for PCR – the longer the oligonucleotide, the less chance there is of it binding to sequences other than the desired target sequence under conditions of high stringency.

TLS Online TPP Program

#Id: 7125

#Unit 6. System Physiology – Plant

TLS Online TPP Program

#Id: 7126

#Unit 6. System Physiology – Plant

Like ABA, strigolactones are derived from carotenoid precursors in plastids in a pathway that is conserved up to synthesis of the intermediate carlactone, beyond which strigolactone biosynthesis diverges in a species- specific manner

TLS Online TPP Program

#Id: 7127

#Unit 6. System Physiology – Plant

TLS Online TPP Program

#Id: 7128

#Unit 6. System Physiology – Plant

Strigolactone biosynthetic pathway and signaling proteins. All-trans-b-carotene is isomerized to 9-cis-b-carotene by a b-carotene isomerase. 9-cis-b-Carotene is cleaved by carotenoid cleavage dioxygenases, to produce carlactone. 

The final stages of strigolactone synthesis and signalling occur in the cytosol.

TLS Online TPP Program

#Id: 7129

#Unit 6. System Physiology – Plant

Strigolactones act locally to repress axillary bud growth

TLS Online TPP Program

#Id: 7130

#Unit 6. System Physiology – Plant

Strigolactones are perceived by a protein complex containing an a-/b-fold hydrolase protein and an F-box protein (D14 and MAX2, respectively)