The PCR primers must not have self complementary regions as this would lead to hairpin formation within primer molecules and make then unsuitable for PCR.

TLS Online TPP Program

#Id: 8626

#Unit 2. Cellular Organization

RBX1 is a RING finger–containing protein that binds the E2 ubiquitin-conjugating enzyme.

TLS Online TPP Program

#Id: 8627

#Unit 2. Cellular Organization

phosphoserine/threonine-binding F-box protein is Skp2, which uses a series of leucine-rich repeats to bind to target proteins only when they are phosphorylated.

TLS Online TPP Program

#Id: 8628

#Unit 2. Cellular Organization

APC/C consists of 14 core subunits in metazoans and 13 core subunits in yeast.

TLS Online TPP Program

#Id: 8629

#Unit 2. Cellular Organization

APC/C works with substrate-targeting factors called Cdc20 and Cdh1

TLS Online TPP Program

#Id: 8630

#Unit 2. Cellular Organization

TLS Online TPP Program

#Id: 8631

#Unit 2. Cellular Organization

APC/C core is organized into three modules: 

1. a scaffold module 

2. a catalytic module

3. a substrate recognition module