The two primers used in a PCR reaction have identical Tm for successful and specific amplification. 

TLS Online TPP Program

#Id: 8489

#Unit 1. Molecules and their Interaction Relevant to Biology

TLS Online TPP Program

#Id: 9774

#Unit 6. System Physiology – Plant

PTI and fungal pathogen entry into epidermal tissue induces several specific cytochrome P450 enzymes, and this leads to accumulation of the indole glucosinolate 4‐methoxyindol‐3‐ylmethylglucosinolate (4M13G).

TLS Online TPP Program

#Id: 2583

#Unit 7. System Physiology – Animal

In late proximal convoluted tubule

High tubular fluid Cl− concentration is the driving

force for reabsorption

TLS Online TPP Program

#Id: 8611

#Unit 2. Cellular Organization

Nonphosphorylated CDK bound to one of its cyclin partners has minimal but detectable protein kinase activity in vitro, although it may be essentially inactive in vivo.

TLS Online TPP Program

#Id: 5277

#Unit 7. System Physiology – Animal

Large quantities of positive potassium ions diffuse out of the SA node.