The efficiency of amplification declines with an increase in the length of the target sequence.

TLS Online TPP Program

#Id: 7487

#Unit 4. Cell Communication and Cell Signaling

Digestion of antibody 

TLS Online TPP Program

#Id: 7488

#Unit 4. Cell Communication and Cell Signaling

Multiple gene segments exist at heavy (V, D, and J) and light-chain (V and J) loci.

TLS Online TPP Program

#Id: 7489

#Unit 4. Cell Communication and Cell Signaling

Junctional diversity- P and N nucleotides  addition 

TLS Online TPP Program

#Id: 7490

#Unit 4. Cell Communication and Cell Signaling

P nucleotide addition results when the DNA hairpin at the coding joint is cleaved asymmetrically.

TLS Online TPP Program

#Id: 7491

#Unit 4. Cell Communication and Cell Signaling

Non - templated N nucleotide addition in heavy chains results from TdT activity. 

TLS Online TPP Program

#Id: 7492

#Unit 4. Cell Communication and Cell Signaling

Exonuclease trimming sometimes occurs at the VDJ and VJ junctions, causing loss of nucleotides.