Fluorescent probes are commonly used to detect rapid biochemical changes in single living cells

TLS Online TPP Program

#Id: 8943

#Unit 2. Cellular Organization

Inactive Tem1-GDP associates with the spindle pole body nearest to the bud as soon as the mitotic spindle forms

TLS Online TPP Program

#Id: 8944

#Unit 2. Cellular Organization

If chromosome segregation occurs properly, extension of the spindle microtubules inserts the daughter SPB into the bud, causing Tem1 to be activated by an unknown mechanism.

TLS Online TPP Program

#Id: 8945

#Unit 2. Cellular Organization

Tem1-GTP activates a protein kinase cascade, which promotes the release of active Cdc14 from the nucleolus and exit from mitosis. 

TLS Online TPP Program

#Id: 8946

#Unit 2. Cellular Organization

An inhibitor of the GTPase, called Kin4, localizes to the parent cell but is absent from the bud.

TLS Online TPP Program

#Id: 8947

#Unit 2. Cellular Organization

An inhibitor of Kin4, called Lte1, localizes to the bud but is absent from the parent cell; it inhibits any residual Kin4 that leaks into the bud.

TLS Online TPP Program

#Id: 8948

#Unit 2. Cellular Organization

When spindle microtubule elongation at the end of anaphase has correctly positioned segregating daughter chromosomes in the bud, Tem1 is converted into its active GTP-bound state, activating the protein kinase signaling cascade.