A modification of overlap extension PCR may also be used to construct directed mutations; this is termed megaprimer PCR .

TLS Online TPP Program

#Id: 6563

#Unit 7. System Physiology – Animal

The glucagon receptor, found primarily on liver cells, is coupled to the Gαs protein, to activate adenylyl cyclase and increase intracellular cAMP

TLS Online TPP Program

#Id: 6564

#Unit 7. System Physiology – Animal

The binding of glucagon to its receptor, leading to activation of protein kinase A, which inhibits glycogen synthesis and promotes glycogenolysis

TLS Online TPP Program

#Id: 6565

#Unit 7. System Physiology – Animal

TLS Online TPP Program

#Id: 6566

#Unit 7. System Physiology – Animal

Glucagon receptors located on the same hepatic cells to activate phospholipase C, and the resulting increase in cytoplasmic Ca2+ also stimulates glycogenolysis

TLS Online TPP Program

#Id: 6567

#Unit 7. System Physiology – Animal

Pancreatic B cells contain GABA, and coincident with the increased insulin secretion produced by hyperglycemia, GABA is released and acts on the A cells by activating GABA A receptors

TLS Online TPP Program

#Id: 6568

#Unit 7. System Physiology – Animal

GABA A receptors are Cl– channels, and the resulting Cl– influx hyperpolarizes the A cells to inhibit glucagon secretion