A modification of overlap extension PCR may also be used to construct directed mutations; this is termed megaprimer PCR .

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#Unit 5. Developmental Biology

Stages of commitment- 

1- Specification 

2- Determination

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#Unit 5. Developmental Biology

Cell Specification

cells become specified to a specific fate

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#Unit 5. Developmental Biology

Specification (capable of being altered)

It is a labile phase, so it is not fixed.

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#Unit 5. Developmental Biology

There are three different modes of cell specification: 

1. Autonomous

2. Conditional

3. Syncytial

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#Unit 5. Developmental Biology

Autonomous Specification

Cell “knows” very early what it is to become without interacting with other cells

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#Unit 5. Developmental Biology

Autonomously specified cells capable of differentiating autonomously (i.e., by itself) when placed in an environment that is neutral with respect to the developmental pathway.