Sometimes the S1 nuclease will not digest the single stranded regions completely, so the transcript appears to be a little longer than it really is, But another method, called primer extension, can tell the 5’-end (but not the 3’-end) to the exact nucleotide

TLS Online TPP Program

#Id: 1859

#Unit 3. Fundamental Processes

Sp1 (Mr  80,000) is a transcription activator for a large number of genes in higher eukaryotes. Its DNA binding site, the GC box consensus sequence GGGCGG.

TLS Online TPP Program

#Id: 1860

#Unit 3. Fundamental Processes

Sp1 contains three zinc fingers, 25% of their amino acid residues are Gln.

TLS Online TPP Program

#Id: 1861

#Unit 3. Fundamental Processes

RNA polymerase III transcribes a set of short genes. The classical class III genes (types I and II) have promoters that lie wholly within the genes.

 

TLS Online TPP Program

#Id: 1862

#Unit 3. Fundamental Processes

RNA Polymerase I has a Bipartite Promoter

 

TLS Online TPP Program

#Id: 1981

#Unit 3. Fundamental Processes

This structure is called a cap.

TLS Online TPP Program

#Id: 1982

#Unit 3. Fundamental Processes

After transcription initiation, Pol II is paused 30 nucleotides downstream from the initiation site, recruits of the capping enzymes.