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A variation on the run-off theme of quantifying accurate transcription in vitro is the G-less cassette assay.

#Unit 13. Methods in Biology #Analysis of RNA #Part B Pointers
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#Unit 6. System Physiology – Plant

ABRE PyACGTGGC & coupling element (CE) is a major cis‐acting element in ABA responsive gene expression. 
The basic leucine zipper (bZIP) transcription factors AREB and ABF can bind to the ABRE cisacting element to activate ABA‐dependent gene expression

TLS Online TPP Program

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#Unit 6. System Physiology – Plant

DREB2A target genes are also induced by dehydration stress

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#Unit 6. System Physiology – Plant

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#Unit 6. System Physiology – Plant

GA stimulates onset of PCD in aleurone layers of barley (Hordeum vulgare) and wheat (Triticum aestivum), ABA postpones PCD

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#Unit 6. System Physiology – Plant

ABA is reported to promote accumulation of H2O2 in leaf tissues and enhance gene expression and enzyme activity for SOD, APX, and CAT

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#Unit 6. System Physiology – Plant

ABA also regulate the NHX1 Na+/H+ antiporter and
other ion transporters during salt stress

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