A variation on the run-off theme of quantifying accurate transcription in vitro is the G-less cassette assay.

TLS Online TPP Program

#Id: 8380

#Unit 2. Cellular Organization

single head domain

Function- Membrane association, endocytosis

TLS Online TPP Program

#Id: 8381

#Unit 2. Cellular Organization

Myosin II

Two heads and assembles into bipolar filaments

Small and non-processive

Function- Contraction

TLS Online TPP Program

#Id: 8382

#Unit 2. Cellular Organization

Myosin V

Two heads but does not assemble into filaments

Large and processive

Function- Organelle transport

TLS Online TPP Program

#Id: 8383

#Unit 2. Cellular Organization

All Myosins move toward the (+) end of an actin filament except myosin VI. Myosin VI move toward the (−) end of an actin filament

TLS Online TPP Program

#Id: 8384

#Unit 2. Cellular Organization

The length of the myosin II neck domain determines the rate of movement.

TLS Online TPP Program

#Id: 8385

#Unit 2. Cellular Organization

In the absence of ATP, the myosin head is firmly attached to the actin filament.