You can use S1 mapping or primer extension analysis to do this measurement, but you can also substitute a reporter gene for the natural gene, and then assay the activity of the reporter gene product.

TLS Online TPP Program

#Id: 7062

#Unit 3. Fundamental Processes

In a phenomenon called codon bias, some codons for a particular amino acid are used more frequently (sometimes much more frequently) than others. A minimum of 32 tRNAs are required to translate all 61 codons (31 to encode the amino acids and 1 for initiation) due to wobble positioning

TLS Online TPP Program

#Id: 7063

#Unit 3. Fundamental Processes

TLS Online TPP Program

#Id: 7064

#Unit 3. Fundamental Processes

TLS Online TPP Program

#Id: 7065

#Unit 3. Fundamental Processes

The addition of the 3’-CCA is catalyzed by the enzyme tRNA nucleotidyltransferase, which functions as a nontemplate-directed RNA polymerase.

TLS Online TPP Program

#Id: 7066

#Unit 3. Fundamental Processes

Multiple tRNAs representing the same amino acid are called isoaccepting tRNAs; because they are all recognized by the same synthetase, they are also described as its cognate tRNAs.

TLS Online TPP Program

#Id: 7067

#Unit 3. Fundamental Processes