AFLP uses restriction enzyme-digested genomic DNA as template for PCR amplification using primers that contain the restriction enzyme recognition sites plus a number of, usually 2-3, arbitrary nucleotides.

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#Id: 4611

#Unit 13. Methods in Biology

FITC binds to Annexin V to phosphatidyl serine which are found on apoptotic cells, analyzed by FACS

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#Id: 4612

#Unit 13. Methods in Biology

The TUNEL Assay measures Apoptotically Generated DNA Fragmentation.

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#Id: 4613

#Unit 13. Methods in Biology

TUNEL method uses TdT to add BrdU to fixed and permeabilized cells which can only incorporated into fragmented DNA of apoptotic cells.

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#Id: 4614

#Unit 13. Methods in Biology

BrdU is incorporated into the newly synthesized DNA, and is then detected with fluorescently labeled anti-BrdU antibodies

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#Id: 4615

#Unit 13. Methods in Biology

Caspases western blotting assay is also used to detect apoptosis

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#Id: 4616

#Unit 13. Methods in Biology

The total concentration of antibody in the equilibrium dialysis chamber is known, the equilibrium equation can be rewritten as: