Linkers are fused with blunt-ended DNA fragments; cleavage of the linker with the appropriate restriction enzyme creates suitable cohesive protruding ends.

TLS Online TPP Program

#Id: 6411

#Unit 2. Cellular Organization

Attenuator riboswitch (Leader sequence) region 1 is translated atleast till codon 10 and 11 in case of Low trp concentration because ribosomes get stalled due to low availability of charged tRNA-trp

TLS Online TPP Program

#Id: 6412

#Unit 2. Cellular Organization

In case of Low trp concentration, RNA polymerase continues to EDCBA genes where new ribosome get attached to AUG of polycistronic genes

TLS Online TPP Program

#Id: 6413

#Unit 2. Cellular Organization

on attenuation for their control. In the case of the leucine operon, its leader peptide has four adjacent leucine codons, and the histidine operon leader peptide has seven histidine codons in a row.

TLS Online TPP Program

#Id: 6414

#Unit 2. Cellular Organization

TLS Online TPP Program

#Id: 6415

#Unit 2. Cellular Organization

RNA Polymerase don’t get dissociated with one hairpin which is 2:3

TLS Online TPP Program

#Id: 6416

#Unit 2. Cellular Organization

Transcription is initiated at the beginning of the 162 nucleotide mRNA leader encoded by a DNA region called trpL