Footprinting is a means of finding the target DNA sequence, or binding site, of a DNA-binding protein.

TLS Online TPP Program

#Id: 7454

#Unit 5. Developmental Biology

In D. discoideum cAMP phosphodiesterase RegA plays a crucial role in regulating intracellular cAMP levels cells & level of PKA activity.

TLS Online TPP Program

#Id: 7455

#Unit 5. Developmental Biology

RegA is activated by phosphorylation of an aspartate in its response regulator domain.

TLS Online TPP Program

#Id: 7456

#Unit 5. Developmental Biology

Phosphorylation of RegA is mediated by sensor histidine kinases/phosphatases which either phosphorylate or dephosphorylate themselves on a histidine residue when a signal molecule binds to their sensor domain.

TLS Online TPP Program

#Id: 7457

#Unit 5. Developmental Biology

TLS Online TPP Program

#Id: 7458

#Unit 5. Developmental Biology

The binding of ammonia to the sensor domain of DhkC leads to the activation of its histidine kinase activity and to the autophosphorylation of a DhkC histidine residue.

TLS Online TPP Program

#Id: 7459

#Unit 5. Developmental Biology

The phosphoryl group is transferred,  From histidine residue of DhkC  aspartate of DhkC histidine in phospho-transfer intermediate RdeA aspartate of RR of RegA