Methylation interference assay Dimethyl sulphate (DMS) methylates purines on DNA strand. Methylate DNA prior to adding protein. Protein binding is prevented at methylated sites. After treatment and removal of protein of interest, treat with piperidine or NaOH which cuts strand at modified bases.
#Unit 13. Methods in Biology#DNA sequencing methods- Footprinting Assays#Part B Pointers