Methylation interference assay Dimethyl sulphate (DMS) methylates purines on DNA strand. Methylate DNA prior to adding protein. Protein binding is prevented at methylated sites. After treatment and removal of protein of interest, treat with piperidine or NaOH which cuts strand at modified bases.

TLS Online TPP Program

#Id: 1689

#Unit 4. Cell Communication and Cell Signaling

Light-chain recombination occurs at the small pre-B-cell stage of B-cell development.

TLS Online TPP Program

#Id: 1690

#Unit 4. Cell Communication and Cell Signaling

Site of DNA cleavage- Recombination Signal Sequence (RSS) 

TLS Online TPP Program

#Id: 1691

#Unit 4. Cell Communication and Cell Signaling

TLS Online TPP Program

#Id: 1692

#Unit 4. Cell Communication and Cell Signaling

Recognition of Recombination Signal Sequence (RSS) by the RAG1/RAG2 enzyme complex.

TLS Online TPP Program

#Id: 1693

#Unit 4. Cell Communication and Cell Signaling

RAG1 is expressed at all phases of the cell cycle, RAG2 is stable only in G0- or G1-phase cells.

TLS Online TPP Program

#Id: 1694

#Unit 4. Cell Communication and Cell Signaling

Threonine residue T490 on RAG2 is phosphorylated during the S, G2, and M phases of the cell cycle, and this phosphorylation triggers the destruction of RAG2.