Reversed-phase liquid chromatography differs from most other forms of chromatography in that the stationary phase is essentially inert and only non-polar (hydrophobic) interactions are possible with analytes.

TLS Online TPP Program

#Id: 2622

#Unit 13. Methods in Biology

Characteristics of the different types of endonucleases.

TLS Online TPP Program

#Id: 2623

#Unit 13. Methods in Biology

The recognition sequences for Type II endonucleases form palindromes with rotational symmetry

TLS Online TPP Program

#Id: 2624

#Unit 13. Methods in Biology

Type III restriction endonucleases are intermediate between the type I and type II enzymes; they cleave DNA in the immediate vicinity of their recognition sites.

TLS Online TPP Program

#Id: 2625

#Unit 13. Methods in Biology

Traditional method of labeling DNA is by the process of nick translation . 

TLS Online TPP Program

#Id: 2626

#Unit 13. Methods in Biology

Steps of nick translation- 

Low concentrations of DNase I are used to make occasional single-strand nicks in the double-stranded DNA that is intended as the gene probe.

DNA polymerase then fills in the nicks, using an appropriate dNTP, at the same time making a new nick to the 3′ side of the previous one.

TLS Online TPP Program

#Id: 2627

#Unit 13. Methods in Biology

Types and examples of typical enzymes used in the manipulation of nucleic acids