Proteins differ in the amount of hydrophobic amino acids that are present on their surface. This difference can be exploited in salt fractionation but can also be used in a higher resolution method using hydrophobic interaction chromatography (HIC).

TLS Online TPP Program

#Id: 4634

#Unit 13. Methods in Biology

Colorimetric Assays for Cell Division Are Rapid and Eliminate the Use of Radioactive Isotopes

TLS Online TPP Program

#Id: 4635

#Unit 13. Methods in Biology

Colorimetric Assays for Cell Division Are Rapid and Eliminate the Use of Radioactive Isotopes

TLS Online TPP Program

#Id: 4636

#Unit 13. Methods in Biology

Bromodeoxyuridine-Based Assays for Cell Division Use Antibodies to Detect Newly Synthesized DNA

TLS Online TPP Program

#Id: 4637

#Unit 13. Methods in Biology

Caspases western blotting assay is also used to detect apoptosis

TLS Online TPP Program

#Id: 4639

#Unit 13. Methods in Biology

Flow cytometry or FACS is the sine qua non (without which, nothing) of the modern immunologist’s toolbox.

Flow cytometry is based on light scattering and fluorescence 

Forward light scattering detect shape and side scattering detects granularity and complexity of the cell

TLS Online TPP Program

#Id: 4640

#Unit 13. Methods in Biology

 Hydrodynamically focused stream separate cells in single file