Proteins differ in the amount of hydrophobic amino acids that are present on their surface. This difference can be exploited in salt fractionation but can also be used in a higher resolution method using hydrophobic interaction chromatography (HIC).

TLS Online TPP Program

#Id: 897

#Unit 13. Methods in Biology

Process of replication in which gap is created by DNase I, filled by klenow fragment and with radioactive dNTPs called as Nick Translation

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#Id: 898

#Unit 13. Methods in Biology

Terminal transferase (TdT) Adds homopolymer tails to the 3’end of DNA.

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#Id: 899

#Unit 13. Methods in Biology

T4 DNA ligase Links 5-phosphate and 3-hydroxyl ends via phosphodiester bond.

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#Id: 900

#Unit 13. Methods in Biology

T4 polynucleotide kinase (PNK) Transfers terminal phosphate groups from ATP to 5-OH groups.

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#Id: 901

#Unit 13. Methods in Biology

Alkaline phosphatase Removes 5’ phosphates from DNA and RNA.

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#Id: 902

#Unit 13. Methods in Biology

Flow cytometry or FACS is the sine qua non (without which, nothing) of the modern immunologist’s toolbox.