Principle of purification of an enzyme by affinity chromatography.

TLS Online TPP Program

#Id: 3966

#Unit 2. Cellular Organization

In E.coli, Protein HU is a dimer that condenses DNA, possibly wrapping it into a beadlike structure.

TLS Online TPP Program

#Id: 3967

#Unit 2. Cellular Organization

The bacterial genome consists of a large number of loops of duplex DNA (in the form of a fiber), each of which is secured at the base to form an independent structural domain.

TLS Online TPP Program

#Id: 3968

#Unit 2. Cellular Organization

Each domain consists of -40 kilobases of DNA, or may be smaller, 10 kb each. About -400 domains in the E. coli genome. The ends of the domains appear to be randomly distributed instead of located at predetermined sites on the chromosome.

TLS Online TPP Program

#Id: 3969

#Unit 2. Cellular Organization

An unrestrained supercoil in the DNA path creates tension, but no tension is transmitted along DNA when a supercoil is restrained by protein binding.

TLS Online TPP Program

#Id: 3970

#Unit 2. Cellular Organization

Chromosomal packaging is maintained by supercoiling of DNA in case of bacteria

TLS Online TPP Program

#Id: 3971

#Unit 2. Cellular Organization

Because supercoiled DNA molecules are more compact than relaxed molecules, they migrate more rapidly during gel electrophoresis