Nurturing Life Sciences
#Id: 2863
The electronic transitions of the peptide bond occur in the far UV. The intense peak at 190 nm, and the weaker one at 210–220 nm is due to the p ® p* and n ® p* transitions.
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#Unit 13. Methods in Biology
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#Id: 4663
TUNEL method uses TdT to add BrdU to fixed and permeabilized cells which can only incorporated into fragmented DNA of apoptotic cells.
#Id: 4664
BrdU is incorporated into the newly synthesized DNA, and is then detected with fluorescently labeled anti-BrdU antibodies
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Caspases western blotting assay is also used to detect apoptosis
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