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TLS Online TPP Program

#Id: 2863

The electronic transitions of the peptide bond occur in the far UV. The intense peak at 190 nm, and the weaker one at 210–220 nm is due to the p ® p* and n ® p* transitions.



#Unit 13. Methods in Biology #Chromatography #Part B Pointers
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TLS Online TPP Program

#Id: 8325

#Unit 2. Cellular Organization

Critical concentration (Cc)
minimal concentration of monomers at which filaments form
TLS Online TPP Program

#Id: 8326

#Unit 2. Cellular Organization

The two ends of microfilament polymerize at different rates:
fast-growing end - plus end
slow-growing end - minus end
TLS Online TPP Program

#Id: 8327

#Unit 2. Cellular Organization
TLS Online TPP Program

#Id: 8328

#Unit 2. Cellular Organization

Hydrolysis of the bound nucleotide reduces the binding affinity of the subunit for neighbouring subunits and makes it more likely to dissociate from each end of the filament.
T form that adds to the filament and the D form that leaves.
TLS Online TPP Program

#Id: 8329

#Unit 2. Cellular Organization

Treadmilling
Because the critical concentrations for the two ends are different, at steady state the free ATP–G-actin will be intermediate between Cc+ and Cc - , so the (+)end will grow and the (-)end will lose subunits.
TLS Online TPP Program

#Id: 8330

#Unit 2. Cellular Organization