Alkylating agents are chemicals that donate alkyl groups to other molecules as nitrogen mustard, and methyl and ethyl methane sulfonate (MMS and EMS).

TLS Online TPP Program

#Id: 2277

#Unit 3. Fundamental Processes

Core enzyme without the s subunit could not transcribe viral DNA, yet had no problems with highly nicked calf thymus DNA

TLS Online TPP Program

#Id: 2278

#Unit 3. Fundamental Processes

With s subunit, the holoenzyme worked equally well on both types of DNA

TLS Online TPP Program

#Id: 2279

#Unit 3. Fundamental Processes

Consensus Sequences

TLS Online TPP Program

#Id: 2280

#Unit 3. Fundamental Processes

The carboxy-terminal domain of the a subunit (aCTD) recognizes the Up-element (where present), whereas s regions 2 and 4 recognize the –10 and –35 regions

TLS Online TPP Program

#Id: 2281

#Unit 3. Fundamental Processes

This consensus sequence was derived by aligning 300 sequences known to function as s70 promoters and ascertaining the most common base found at each position in the –35 and –10 hexamers.

TLS Online TPP Program

#Id: 2282

#Unit 3. Fundamental Processes

Note that there is no significant consensus among the 17–19 nucleotides that lie in the region between –35 and –10.