HU has the capacity to bend DNA, and is involved in building the structure that leads to formation of the open complex.

TLS Online TPP Program

#Id: 3024

#Unit 3. Fundamental Processes

 DnaB is the helicase that unwinds DNA for replication in E.coli.

TLS Online TPP Program

#Id: 3025

#Unit 3. Fundamental Processes

A preformed RNA (often a tRNA) pairs with the template, allowing its 3'-0H end to be used to prime DNA synthesis.

TLS Online TPP Program

#Id: 3026

#Unit 3. Fundamental Processes

Priming activity is required to provide 3'-0H ends to start off the DNA chains on both the leading and lagging strands.

TLS Online TPP Program

#Id: 3027

#Unit 3. Fundamental Processes

Each Okazaki fragment starts with a primer sequence of RNA - 10 bases long that provides the 3'-0H end for extension by DNA polymerase.

TLS Online TPP Program

#Id: 3028

#Unit 3. Fundamental Processes

Primers start with the sequence pppAG positioned opposite the sequence 3 '-GTC-5' in the template.

TLS Online TPP Program

#Id: 3029

#Unit 3. Fundamental Processes

The oriCsystem, named for the bacterial origin, basically involves the association of the DnaG primase with the protein complex at the replication fork.