Once the prepriming complex is loaded onto the replication forks, the next step is the recruitment of the primase, DnaG, which is then loaded onto the DnaB hexamer.

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#Id: 3807

#Unit 5. Developmental Biology

CMF stimulates gene expression in parallel with PSF, and both signals potentiate cAMP signalling by inducing genes involved in cAMP synthesis and detection

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#Id: 3808

#Unit 5. Developmental Biology

Newly activated genes are:

Adenylate cyclase A (ACA)

PKA

Extracellular cAMP phosphodiesterase PdsA

An intracellular cAMP phosphodiesterase RegA

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#Id: 3809

#Unit 5. Developmental Biology

Once all proteins are functioning, a few starving cells start to emit cAMP pulses.

TLS Online TPP Program

#Id: 3811

#Unit 5. Developmental Biology

Chemotaxis of Dictyostelium myxamoebae is a result of spiral waves of cAMP.

TLS Online TPP Program

#Id: 3817

#Unit 5. Developmental Biology

Neighboring cells respond to cAMP in two ways:

Initiate a movement toward the cAMP pulse for about a minute

Release cAMP of their own

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#Id: 3820

#Unit 5. Developmental Biology

The top of the mound continues to emit cAMP pulses and is pushed upwards by the movement of cells underneath