Mutations in three adjacent elements, numbered B 1 to B3, reduce origin function.

TLS Online TPP Program

#Id: 380

#Unit 2. Cellular Organization

The histone-fold domain is composed of three a-helical regions separated by two short unstructured loops.

 

TLS Online TPP Program

#Id: 381

#Unit 2. Cellular Organization

Histone-fold domain mediates the assembly of Histone-only Intermediates

TLS Online TPP Program

#Id: 382

#Unit 2. Cellular Organization

H3 and H4 histones first form heterodimers that then come together to form a tetramer with two molecules each of H3 and H4. While H2A and H2B form heterodimers in solution but not tetramers. This assembly without DNA called Histone-only Intermediates 

TLS Online TPP Program

#Id: 383

#Unit 2. Cellular Organization

First the H3.H4 assemble into tetramer (HOI) then DNA wrapped over H3.H4 tetramer. 

TLS Online TPP Program

#Id: 384

#Unit 2. Cellular Organization

TLS Online TPP Program

#Id: 385

#Unit 2. Cellular Organization

H32-H42 tetramer accounts for the diameter of the octamer and H32-H42 tetramer alone can organize DNA in vitro into particles that display the properties of the core particle.