Diversity within species 

450-700 (mycoplasma)

4000 in bacteria (eg. Escherichia coli)

13,000 in Fruit-fly (Drosophila melanogaster)

32,000 – 50,000 in rice (Oryza sativa)

35,000 to 45,000 in human beings (Homo sapiens sapiens). 

TLS Online TPP Program

#Id: 8769

#Unit 1. Molecules and their Interaction Relevant to Biology

TLS Online TPP Program

#Id: 8770

#Unit 1. Molecules and their Interaction Relevant to Biology

                                                                                                                                                                                                                                                                      

TLS Online TPP Program

#Id: 8771

#Unit 1. Molecules and their Interaction Relevant to Biology

TLS Online TPP Program

#Id: 8772

#Unit 1. Molecules and their Interaction Relevant to Biology

van’t Hoff plot is used measure rate of unfolding of proteins

TLS Online TPP Program

#Id: 8773

#Unit 1. Molecules and their Interaction Relevant to Biology

Techniques for Measuring Stability

Absorbance (e.g. Trp, Tyr) 

Fluorescence (Trp)-difference in emission max & intensity.

CD (far or near UV) - (2o or 3o) 

NMR 

DSC (calorimetry) 

Urea gradient gels - difference in the migrating rates between F and U.

Catalytic activity 

Chromophoric or fluorophoric probes/FRET 

TLS Online TPP Program

#Id: 8774

#Unit 1. Molecules and their Interaction Relevant to Biology

The Cp heat capacity of a defined system is the amount of heat (usually expressed in calories, kilocalories, or joules) needed to raise the system's temperature by one degree (usually expressed in Celsius or Kelvin).