The time of sedimentation (in seconds) for a spherical particle is (Please see the picture below) where t is the sedimentation time, is the viscosity of medium, R hydro is the hydrodynamic radius of the particle, r b is the radial distance from the centre of rotation to bottom of tube, r t is the radial distance from the centre of rotation to liquid meniscus, ρ is the density of the particle, m is the density of the medium and is the angular velocity of the rotor.
#Unit 13. Methods in Biology#Centrifugation#Part B Pointers